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rnr [2019-09-23 12:32:32]
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rnr [2019-09-23 12:32:32]

RNase R, required for protection against paraquate stress
Locus
BSU_33610
Isoelectric point
5.70
Molecular weight
88.56 kDa
Protein length
779 aa Sequence Blast
Gene length
2340 bp Sequence Blast
Function
nonspecific degradation of rRNA
Product
exoribonuclease RNase R (EC 3.1.-.-)
Essential
no
Synonyms
yvaJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of nucleotides/ other
  • Information processing → RNA synthesis and degradation → RNases → Exoribonucleases
  • Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress

    • Gene

    Coordinates
    3,451,863 3,454,202

    The protein

    Catalyzed reaction/ biological activity

  • 3'-5'-exoribonuclease
  • Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates (according to UniProt)

    • Protein family

  • RNR ribonuclease family (single member, according to UniProt)

    • Domains

  • S1 domain (aa 625-708) (according to the Interpro database)
  • 2 CSD domains (aa 73-133, aa 138-202) (according to the Interpro database)

    • Structure

  • 5XGU (from E. coli, 40% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    secG-yvaK-rnr-smpB-ssrA
    Description
    PubMed

    Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon

    • Regulation

  • yvaK: induced by stress (SigB) PubMed
    • view in new tab

    Genes
    yvaK-rnr-smpB-ssrA
    Description
    PubMed

    Regulation

  • yvaK: induced by stress (SigB) PubMed
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A495 (yvaJ::erm), available at the NBRP B. subtilis, Japan
  • BKE33610 (rnr::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCCATTATCCGACCTCCTG, downstream forward: _UP4_TAGCAGCTCAACCAGCAAAT
  • BKK33610 (rnr::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCCATTATCCGACCTCCTG, downstream forward: _UP4_TAGCAGCTCAACCAGCAAAT

    • Labs working on this gene/protein

  • David Bechhofer, Mount Sinai School, New York, USA Homepage

    • References

    Reviews

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    Original publications

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